PLCD4 monoclonal antibody (M01), clone 4D4
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PLCD4.
Immunogen
PLCD4 (AAH06355, 18 a.a. ~ 127 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MQEGMPMRKVRSKSWKKLRYFRLQNDGMTVWHARQARGSAKPSFSISDVETIRNGHDSELLRSLAEELPLEQGFTIVFHGRRSNLDLMANSVEEAQIWMRGLQLLVDLVT
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (82); Rat (79)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PLCD4 expression in transfected 293T cell line by PLCD4 monoclonal antibody (M01), clone 4D4.
Lane 1: PLCD4 transfected lysate (Predicted MW: 87.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PLCD4 is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — PLCD4
Entrez GeneID
84812GeneBank Accession#
BC006355Protein Accession#
AAH06355Gene Name
PLCD4
Gene Alias
MGC12837
Gene Description
phospholipase C, delta 4
Omim ID
605939Gene Ontology
HyperlinkGene Summary
Phosphatidylinositol-specific phospholipase C (PLC) plays an important role in receptor-mediated signal transduction by generating 2 second messenger molecules, inositol 1,4,5-triphosphate (IP3) and diacylglycerol, from phosphatidylinositol 4,5-bisphosphate (PIP2). PLC comprises a diverse family of enzymes that differ in structure and tissue distribution (Berridge, 1993 [PubMed 8381210]).[supplied by OMIM
Other Designations
PLC delta4
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Interactome
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Pathway
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Disease
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