GPT2 monoclonal antibody (M04), clone 7A11
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of GPT2 expression in transfected 293T cell line by GPT2 monoclonal antibody (M04), clone 7A11.
Lane 1: GPT2 transfected lysate (Predicted MW: 57.9 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GPT2 is 1 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to GPT2 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.63 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant GPT2.
Immunogen
GPT2 (NP_597700, 358 a.a. ~ 456 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
NLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVAGEESFEQFSREKESVLGNLAKKAKLTEDLFNQVPGIHCNPLQGAMYAFPRIFIPAKAVEAAQ
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (95); Rat (96)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of GPT2 expression in transfected 293T cell line by GPT2 monoclonal antibody (M04), clone 7A11.
Lane 1: GPT2 transfected lysate (Predicted MW: 57.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GPT2 is 1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to GPT2 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — GPT2
Entrez GeneID
84706GeneBank Accession#
NM_133443Protein Accession#
NP_597700Gene Name
GPT2
Gene Alias
ALT2
Gene Description
glutamic pyruvate transaminase (alanine aminotransferase) 2
Omim ID
138210Gene Ontology
HyperlinkGene Summary
GPT (MIM 138200) and GPT2 (EC 2.6.1.2), also known as alanine transaminases, are pyridoxal enzymes that catalyze the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. By mediating the conversion of these 4 major intermediate metabolites, these transaminases have roles in gluconeogenesis and in amino acid metabolism.[supplied by OMIM
Other Designations
alanine aminotransferase 2|glutamic pyruvate transaminase 2|glutamic--alanine transaminase|glutamic--pyruvic transaminase|glutamic-pyruvate transaminase 2
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Interactome
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Pathway
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