TRIM63 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
TRIM63 transfected lysate.
Western Blot
Lane 1: TRIM63 transfected lysate ( 40.20 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TRIM63 full-length
Host
Human
Theoretical MW (kDa)
40.2
Interspecies Antigen Sequence
Mouse (93); Rat (92)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TRIM63 antibody (H00084676-D01P) by Western Blots.
SDS-PAGE Gel
TRIM63 transfected lysate.
Western Blot
Lane 1: TRIM63 transfected lysate ( 40.20 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TRIM63
Entrez GeneID
84676GeneBank Accession#
NM_032588.2Protein Accession#
NP_115977.2Gene Name
TRIM63
Gene Alias
FLJ32380, IRF, MURF1, MURF2, RNF28, SMRZ
Gene Description
tripartite motif-containing 63
Omim ID
606131Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle. [provided by RefSeq
Other Designations
OTTHUMP00000008701|iris ring finger protein|muscle specific ring finger protein 1|muscle specific ring finger protein 2|ring finger protein 28|striated muscle RING zinc finger protein
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