GNPTG 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GNPTG transfected lysate.
Western Blot
Lane 1: GNPTG transfected lysate ( 31.57 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GNPTG full-length
Host
Human
Theoretical MW (kDa)
31.57
Interspecies Antigen Sequence
Mouse (80); Rat (79)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GNPTG antibody (H00084572-B01) by Western Blots.
SDS-PAGE Gel
GNPTG transfected lysate.
Western Blot
Lane 1: GNPTG transfected lysate ( 31.57 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GNPTG
Entrez GeneID
84572GeneBank Accession#
BC014592Protein Accession#
AAH14592Gene Name
GNPTG
Gene Alias
C16orf27, GNPTAG, LP2537, RJD9
Gene Description
N-acetylglucosamine-1-phosphate transferase, gamma subunit
Gene Ontology
HyperlinkGene Summary
UDP-N-acetylglucosamine:lysosomal-enzyme N-acetylglucosamine-1-phosphotransferase (GlcNAc-phosphotransferase) catalyzes the initial step in the synthesis of the mannose 6-phosphate determinant required for efficient intracellular targeting of newly synthesized lysosomal hydrolases to the lysosome. GlcNAc-phosphotransferase is an alpha-2/beta-2/gamma-2 hexameric complex, the enzyme product of 2 genes, 1 encoded by a gene on chromosome 16 (GNPTG) and the other by a gene on chromosome 12 (GNPTAB; MIM 607840).[supplied by OMIM
Other Designations
GlcNAc-phosphotransferase gamma-subunit|N-acetylglucosamine-1-phosphotransferase, gamma subunit
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