DOT1L monoclonal antibody (M01), clone 6A6
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More Files
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Western Blot (Cell lysate)
DOT1L monoclonal antibody (M01), clone 6A6. Western Blot analysis of DOT1L expression in LNCaP.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DOT1L is approximately 0.03ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to DOT1L on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (37.4 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant DOT1L.
Immunogen
DOT1L (NP_115871, 3 a.a. ~ 108 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
EKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLR
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (99); Rat (91)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.4 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
DOT1L monoclonal antibody (M01), clone 6A6. Western Blot analysis of DOT1L expression in LNCaP.Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DOT1L is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to DOT1L on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — DOT1L
Entrez GeneID
84444GeneBank Accession#
NM_032482Protein Accession#
NP_115871Gene Name
DOT1L
Gene Alias
DKFZp586P1823, DOT1, KIAA1814, KMT4
Gene Description
DOT1-like, histone H3 methyltransferase (S. cerevisiae)
Omim ID
607375Gene Ontology
HyperlinkGene Summary
O
Other Designations
DOT1-like, histone H3 methyltransferase|histone methyltransferase DOT1L
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Interactome
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Pathway
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Disease
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Publication Reference
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Incidence of methylated histones H3K4 and H3K79 in cat germinal vesicles is regulated by specific nuclear factors at the acquisition of developmental competence during the folliculogenesis.
Phillips TC, Wildt DE, Comizzoli P.
Journal of Assisted Reproduction and Genetics 2016 Apr; 33(6):783.
Application：IF, Cat, ovary.
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Incidence of methylated histones H3K4 and H3K79 in cat germinal vesicles is regulated by specific nuclear factors at the acquisition of developmental competence during the folliculogenesis.
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