PCGF5 monoclonal antibody (M01), clone 3C10
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PCGF5 expression in transfected 293T cell line by PCGF5 monoclonal antibody (M01), clone 3C10.
Lane 1: PCGF5 transfected lysate(29.7 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PCGF5 is 0.3 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to PCGF5 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.52 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PCGF5.
Immunogen
PCGF5 (NP_115749.2, 51 a.a. ~ 148 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
NDCPRCGNQVHETNPLEMLRLDNTLEEIIFKLVPGLREQELERESEFWKKNKPQENGQDDTSKADKPKVDEEGDENEDDKDYHRSDPQIAICLDCLRN
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (93); Rat (92)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.52 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PCGF5 expression in transfected 293T cell line by PCGF5 monoclonal antibody (M01), clone 3C10.
Lane 1: PCGF5 transfected lysate(29.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PCGF5 is 0.3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PCGF5 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PCGF5
Entrez GeneID
84333GeneBank Accession#
NM_032373Protein Accession#
NP_115749.2Gene Name
PCGF5
Gene Alias
MGC16202, RNF159
Gene Description
polycomb group ring finger 5
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000020085|ring finger protein (C3HC4 type) 159
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Interactome
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Disease
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