MGC10540 (Human) Recombinant Protein (P01)
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Specification
Product Description
Human MGC10540 full-length ORF ( AAH06282, 1 a.a. - 176 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVKLQRKLPVESIQIVLEELRKKGNLEWLDKSKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEEFHGLDEATLLRALQALQQEHKAEIITVSDGRGVKFF
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
45.1
Interspecies Antigen Sequence
Mouse (95); Rat (98)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — VPS25
Entrez GeneID
84313GeneBank Accession#
BC006282Protein Accession#
AAH06282Gene Name
VPS25
Gene Alias
DERP9, EAP20, FAP20, MGC10540
Gene Description
vacuolar protein sorting 25 homolog (S. cerevisiae)
Omim ID
610907Gene Ontology
HyperlinkGene Summary
VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM
Other Designations
vacuolar protein sorting 25
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Interactome
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Pathway
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Disease
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