VPS25 monoclonal antibody (M01), clone 2E5-2B9
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More Files
- More Functions
Western Blot (Cell lysate)
MGC10540 monoclonal antibody (M01), clone 2E5-2B9 Western Blot analysis of MGC10540 expression in Hela S3 NE ( Cat # L013V3 ).
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged VPS25 is approximately 0.03ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to VPS25 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (45.1 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant VPS25.
Immunogen
VPS25 (AAH06282, 1 a.a. ~ 176 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVKLQRKLPVESIQIVLEELRKKGNLEWLDKSKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEEFHGLDEATLLRALQALQQEHKAEIITVSDGRGVKFF
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (95); Rat (98)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (45.1 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
MGC10540 monoclonal antibody (M01), clone 2E5-2B9 Western Blot analysis of MGC10540 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged VPS25 is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to VPS25 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — VPS25
Entrez GeneID
84313GeneBank Accession#
BC006282Protein Accession#
AAH06282Gene Name
VPS25
Gene Alias
DERP9, EAP20, FAP20, MGC10540
Gene Description
vacuolar protein sorting 25 homolog (S. cerevisiae)
Omim ID
610907Gene Ontology
HyperlinkGene Summary
VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM
Other Designations
vacuolar protein sorting 25
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Interactome
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Pathway
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Disease
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