MORG1 monoclonal antibody (M02), clone 4E12
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MORG1.
Immunogen
MORG1 (NP_115708, 171 a.a. ~ 262 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
VDGRVRRYDLRMGQLFSDYVGSPITCTCFSRDGQCTLVSSLDSTLRLLDKDTGELLGEYKGHKNQEYKLDCCLSERDTHVVSCSEDGKVFFW
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (95); Rat (95)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.86 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MORG1 expression in transfected 293T cell line by MORG1 monoclonal antibody (M02), clone 4E12.
Lane 1: MORG1 transfected lysate (Predicted MW: 34.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MORG1 is 1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to MORG1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — MORG1
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Interactome
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Publication Reference
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MORG1 limits mTORC1 signaling by inhibiting Rag GTPases.
Yakubu Princely Abudu, Athanasios Kournoutis, Hanne Britt Brenne, Trond Lamark, Terje Johansen.
Molecular Cell 2024 Feb; 84(3):552.
Application:N/A, N/A, N/A.
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Morg1(+/-) heterozygous mice are protected from experimentally induced focal cerebral ischemia.
Stahr A, Frahm C, Kretz A, Bondeva T, Witte OW, Wolf G.
Brain Research 2012 Oct; 1482:22.
Application:IHC-Fr, Mouse, Mouse coronal sections.
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MORG1 limits mTORC1 signaling by inhibiting Rag GTPases.
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