MAF1 monoclonal antibody (M02), clone 2D6
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant MAF1.
Immunogen
MAF1 (AAH31273.1, 1 a.a. ~ 256 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MKLLENSSFEAINSQLTVETGDAHIIGRIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPSRLSKSQGGEEEGPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLSWVVNAVNCSLFSAVREDFKDLKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCRSISGSTYTPSEAGNELDMELGEEEVEEESRSRGSGAEETSTMEEDRVPVICI
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (55.3 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MAF1 expression in transfected 293T cell line by MAF1 monoclonal antibody (M02), clone 2D6.
Lane 1: MAF1 transfected lysate (Predicted MW: 28.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAF1 is 3 ng/ml as a capture antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAF1 is 1 ng/ml as a capture antibody.ELISA
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Gene Info — MAF1
Entrez GeneID
84232GeneBank Accession#
BC031273.1Protein Accession#
AAH31273.1Gene Name
MAF1
Gene Alias
MGC20332, MGC31779, MGC39758
Gene Description
MAF1 homolog (S. cerevisiae)
Omim ID
610210Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. [provided by RefSeq
Other Designations
MAF1 protein|homolog of yeast MAF1
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