POLR1B rabbit monoclonal antibody
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Specification
Product Description
Rabbit monoclonal antibody raised against a human POLR1B peptide using ARM Technology.
Immunogen
A synthetic peptide of human POLR1B is used for rabbit immunization.
Customer or Abnova will decide on the preferred peptide sequence.Host
Rabbit
Library Construction
Non-fusion antibody library from rabbit spleen (ARM Technology).
Expression
Overexpression vector and transfection into 293H cell line.
Reactivity
Human
Purification
Protein A
Isotype
IgG
Quality Control Testing
Antibody reactive against human POLR1B peptide by ELISA and mammalian transfected lysate by Western Blot.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Deliverable
Up to three rabbit IgG clones of 100 ug each will be delivered to customer.
Note
1. Customer may provide cell or tissue lysate for antibody screening.
2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request. -
Applications
Western Blot (Transfected lysate)
ELISA
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Gene Info — POLR1B
Entrez GeneID
84172GeneBank Accession#
POLR1BGene Name
POLR1B
Gene Alias
FLJ10816, FLJ21921, MGC131780, RPA135, RPA2, Rpo1-2
Gene Description
polymerase (RNA) I polypeptide B, 128kDa
Omim ID
602000Gene Ontology
HyperlinkGene Summary
Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM
Other Designations
DNA-directed RNA polymerase I 135kDa polypeptide|RNA polymerase I polypeptide B|RNA polymerase I subunit 2
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