EIF2A monoclonal antibody (M01), clone 3D5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of EIF2A expression in transfected 293T cell line by EIF2A monoclonal antibody (M01), clone 3D5.
Lane 1: EIF2A transfected lysate(65 KDa).
Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (37.11 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant EIF2A.
Immunogen
EIF2A (NP_114414, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAPSTPLLTVRGSEGLYMVNGPPHFTESTVFPRESGKNCKVCIFSKDGTLFAWGNGEKVNIISVTNKGLLHSFDLLKAVCLEFSPKNTVLATWQPYTTSK
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (91); Rat (80)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.11 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of EIF2A expression in transfected 293T cell line by EIF2A monoclonal antibody (M01), clone 3D5.
Lane 1: EIF2A transfected lysate(65 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
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Gene Info — EIF2A
Entrez GeneID
83939GeneBank Accession#
NM_032025Protein Accession#
NP_114414Gene Name
EIF2A
Gene Alias
CDA02, EIF-2A, MST089, MSTP004, MSTP089
Gene Description
eukaryotic translation initiation factor 2A, 65kDa
Omim ID
609234Gene Ontology
HyperlinkGene Summary
EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 [PubMed 12133843]).[supplied by OMIM
Other Designations
eukaryotic translation initiation factor 2A
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Interactome
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Disease
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Publication Reference
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Protein Kinase R Is Responsible for the Phosphorylation of eIF2{alpha} in Rotavirus Infection.
Rojas M, Arias CF, Lopez S.
Journal of Virology 2010 Jul; 84(20):10457.
Application：WB, Monkey, MA104 cells.
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Protein Kinase R Is Responsible for the Phosphorylation of eIF2{alpha} in Rotavirus Infection.
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