ROPN1L 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ROPN1L transfected lysate.
Western Blot
Lane 1: ROPN1L transfected lysate ( 26.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ROPN1L full-length
Host
Human
Theoretical MW (kDa)
26.2
Interspecies Antigen Sequence
Mouse (79); Rat (78)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ROPN1L antibody (H00083853-B01) by Western Blots.
SDS-PAGE Gel
ROPN1L transfected lysate.
Western Blot
Lane 1: ROPN1L transfected lysate ( 26.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ROPN1L
Entrez GeneID
83853GeneBank Accession#
BC014607Protein Accession#
AAH14607.1Gene Name
ROPN1L
Gene Alias
ASP, FLJ23003, FLJ25776
Gene Description
ropporin 1-like
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a sperm protein, which interacts with A-kinase anchoring protein, AKAP3, through the amphipathic helix region of AKAP3. Type II regulatory subunit of cAMP-dependent protein kinase (PKARII) also binds to this helix domain of AKAP3, which allows PKARII to be targeted to specific subcellular compartments. It is suggested that sperm contains several proteins that bind to AKAPs in a manner similar to PKARII, and this encoded protein may be one of them. [provided by RefSeq
Other Designations
AKAP-associated sperm protein
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