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ITCH 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00083737-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-ITCH full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 94.93
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ITCH antibody (H00083737-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00083737-T01
    ITCH transfected lysate.
    Western Blot
    QC Testing of H00083737-T01
    Lane 1: ITCH transfected lysate ( 94.93 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • ITCH
  • Gene Alias:
  • AIF4,AIP4,NAPP1,dJ468O1.1
  • Gene Description:
  • itchy E3 ubiquitin protein ligase homolog (mouse)
  • Gene Summary:
  • Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells. [provided by RefSeq
  • Other Designations:
  • NFE2-associated polypeptide 1,atrophin-1 interacting protein 4,dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)),itchy homolog E3 ubiquitin protein ligase,ubiquitin protein ligase ITCH
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