RIOK1 purified MaxPab mouse polyclonal antibody (B02P)
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of RIOK1 expression in transfected 293T cell line (H00083732-T03) by RIOK1 MaxPab polyclonal antibody.
Lane 1: RIOK1 transfected lysate(65.6 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to RIOK1 on HeLa cell. [antibody concentration 20 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human RIOK1 protein.
Immunogen
RIOK1 (NP_113668.2, 1 a.a. ~ 568 a.a) full-length human protein.
Sequence
MDYRRLLMSRVVPGQFDDADSSDSENRDLKTVKEKDDILFEDLQDNVNENGEGEIEDEEEEGYDDDDDDWDWDEGVGKLAKGYVWNGGSNPQANRQTSDSSSAKMSTPADKVLRKFENKINLDKLNVTDSVINKVTEKSRQKEADMYRIKDKADRATVEQVLDPRTRMILFKMLTRGIITEINGCISTGKEANVYHASTANGESRAIKIYKTSILVFKDRDKYVSGEFRFRHGYCKGNPRKMVKTWAEKEMRNLIRLNTAEIPCPEPIMLRSHVLVMSFIGKDDMPAPLLKNVQLSESKARELYLQVIQYMRRMYQDARLVHADLSEFNMLYHGGGVYIIDVSQSVEHDHPHALEFLRKDCANVNDFFMRHSVAVMTVRELFEFVTDPSITHENMDAYLSKAMEIASQRTKEERSSQDHVDEEVFKRAYIPRTLNEVKNYERDMDIIMKLKEEDMAMNAQQDNILYQTVTGLKKDLSGVQKVPALLENQVEERTCSDSEDIGSSECSDTDSEEQGDHARPKKHTTDPDIDKKERKKMVKEAQREKRKNKIPKHVKKRKEKTAKTKKGK
Host
Mouse
Reactivity
Human
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of RIOK1 expression in transfected 293T cell line (H00083732-T03) by RIOK1 MaxPab polyclonal antibody.
Lane 1: RIOK1 transfected lysate(65.6 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to RIOK1 on HeLa cell. [antibody concentration 20 ug/ml] -
Gene Info — RIOK1
Entrez GeneID
83732GeneBank Accession#
NM_031480.2Protein Accession#
NP_113668.2Gene Name
RIOK1
Gene Alias
AD034, FLJ30006, MGC12903, bA288G3.1
Gene Description
RIO kinase 1 (yeast)
Gene Ontology
HyperlinkGene Summary
This gene includes two alternatively spliced transcript variants, which encode different isoforms. The function of this gene has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000015999|RIO kinase 1|prematurely truncated due to insertion of intronic sequence
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Interactome
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Publication Reference
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Rio1 downregulates centromeric RNA levels to promote the timely assembly of structurally fit kinetochores.
Ksenia Smurova, Michela Damizia, Carmela Irene, Stefania Stancari, Giovanna Berto, Giulia Perticari, Maria Giuseppina Iacovella, Ilaria D'Ambrosio, Maria Giubettini, Réginald Philippe, Chiara Baggio, Elisabetta Callegaro, Andrea Casagranda, Alessandro Corsini, Vincenzo Gentile Polese, Anna Ricci, Erik Dassi, Peter De Wulf.
Nature Communications 2023 Jun; 14(1):3172.
Application：IF, WB, Human, hTERT-RPE1 cells.
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Rio1 downregulates centromeric RNA levels to promote the timely assembly of structurally fit kinetochores.
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