SESN2 monoclonal antibody (M03), clone 3B8
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant SESN2.
Immunogen
SESN2 (AAH13304.1, 1 a.a. ~ 480 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MIVADSECRAELKDYLRFAPGGVGDSGPGEEQRESRARRGPRGPSAFIPVEEVLREGAESLEQHLGLEALMSSGRVDNLAVVMGLHPDYFTSFWRLHYLLLHTDGPLASSWRHYIAIMAAARHQCSYLVGSHMAEFLQTGGDPEWLLGLHRAPEKLRKLSEINKLLAHRPWLITKEHIQALLKTGEHTWSLAELIQALVLLTHCHSLSSFVFGCGILPEGDADGSPAPQAPTPPSEQSSPPSRDPLNNSGGFESARDVEALMERMQQLQESLLRDEGTSQEEMESRFELEKSESLLVTPSADILEPSPHPDMLCFVEDPTFGYEDFTRRGAQAPPTFRAQDYTWEDHGYSLIQRLYPEGGQLLDEKFQAAYSLTYNTIAMHSGVDTSVLRRAIWNYIHCVFGIRYDDYDYGEVNQLLERNLKVYIKTVACYPEKTTRRMYNLFWRHFRHSEKVHVNLLLLEARMQAALLYALRAITRYMT
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (91); Rat (91)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (78.54 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of SESN2 expression in transfected 293T cell line by SESN2 monoclonal antibody (M03), clone 3B8.
Lane 1: SESN2 transfected lysate(54.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to SESN2 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3 ug/ml]Immunoprecipitation
Immunoprecipitation of SESN2 transfected lysate using anti-SESN2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SESN2 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SESN2 is approximately 0.3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to SES2 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — SESN2
Entrez GeneID
83667GeneBank Accession#
BC013304Protein Accession#
AAH13304.1Gene Name
SESN2
Gene Alias
DKFZp761M0212, DKFZp761M02121, HI95, SES2, SEST2
Gene Description
sestrin 2
Omim ID
607767Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the sestrin family of PA26-related proteins. The encoded protein may function in the regulation of cell growth and survival. This protein may be involved in cellular response to different stress conditions. [provided by RefSeq
Other Designations
OTTHUMP00000003641|hypoxia induced gene 95
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Interactome
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Pathway
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Publication Reference
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Arsenic trioxide-mediated suppression of miR-182-5p is associated with potent anti-oxidant effects through up-regulation of SESN2.
Lin LT, Liu SY, Leu JD, Chang CY, Chiou SH, Lee TC, Lee YJ.
Oncotarget 2018 Mar; 9(22):16028.
Application:WB, Human, S1 GBM, U87MG, Human lung adenocarcinoma H1299, A549 cells.
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The inhibition of autophagy sensitises colon cancer cells with wild-type p53 but not mutant p53 to topotecan treatment.
Li DD, Sun T, Wu XQ, Chen SP, Deng R, Jiang S, Feng GK, Pan JX, Zhang XS, Zeng YX, Zhu XF.
PLoS One 2012 Sep; 7(9):e45058.
Application:WB, Human, HCT-116, LS-174T cells.
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Association of platelet-derived growth factor receptor accumulation with increased oxidative stress and cellular injury in sestrin 2 silenced human glioblastoma cells.
Liu SY, Lee YJ, Lee TC.
FEBS Letters 2011 Jun; 585(12):1853.
Application:WB-Tr, Human, U87 cells.
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Arsenic trioxide-mediated suppression of miR-182-5p is associated with potent anti-oxidant effects through up-regulation of SESN2.
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