DOHH 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
DOHH transfected lysate.
Western Blot
Lane 1: DOHH transfected lysate ( 32.90 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DOHH full-length
Host
Human
Theoretical MW (kDa)
32.9
Interspecies Antigen Sequence
Mouse (85); Rat (83)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DOHH antibody (H00083475-B01P) by Western Blots.
SDS-PAGE Gel
DOHH transfected lysate.
Western Blot
Lane 1: DOHH transfected lysate ( 32.90 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DOHH
Entrez GeneID
83475GeneBank Accession#
NM_031304Protein Accession#
NP_112594Gene Name
DOHH
Gene Alias
HLRC1, MGC4293
Gene Description
deoxyhypusine hydroxylase/monooxygenase
Omim ID
611262Gene Ontology
HyperlinkGene Summary
This gene encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants
Other Designations
HEAT-like (PBS lyase) repeat containing 1
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