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Last updated: 2017/4/30

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DOHH 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00083475-T03
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-DOHH full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 32.9
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-DOHH antibody (H00083475-B01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00083475-T03
    DOHH transfected lysate.
    Western Blot
    QC Testing of H00083475-T03
    Lane 1: DOHH transfected lysate ( 32.90 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • DOHH
  • Gene Alias:
  • HLRC1,MGC4293
  • Gene Description:
  • deoxyhypusine hydroxylase/monooxygenase
  • Gene Summary:
  • This gene encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants
  • Other Designations:
  • HEAT-like (PBS lyase) repeat containing 1
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