DOHH (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human DOHH full-length ORF ( NP_112594.1, 1 a.a. - 302 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MVTEQEVDAIGQTLVDPKQPLQARFRALFTLRGLGGPGAIAWISQAFDDDSALLKHELAYCLGQMQDARAIPMLVDVLQDTRQEPMVRHEAGEALGAIGDPEVLEILKQYSSDPVIEVAETCQLAVRRLEWLQQHGGEPAAGPYLSVDPAPPAEERDVGRLREALLDESRPLFERYRAMFALRNAGGEEAALALAEGLHCGSALFRHEVGYVLGQLQHEAAVPQLAAALARCTENPMVRHECAEALGAIARPACLAALQAHADDPERVVRESCEVALDMYEHETGRAFQYADGLEQLRGAPS
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
59.3
Interspecies Antigen Sequence
Mouse (85); Rat (83)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — DOHH
Entrez GeneID
83475GeneBank Accession#
NM_031304.2Protein Accession#
NP_112594.1Gene Name
DOHH
Gene Alias
HLRC1, MGC4293
Gene Description
deoxyhypusine hydroxylase/monooxygenase
Omim ID
611262Gene Ontology
HyperlinkGene Summary
This gene encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants
Other Designations
HEAT-like (PBS lyase) repeat containing 1
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Interactome
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