QTRT1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
QTRT1 transfected lysate.
Western Blot
Lane 1: QTRT1 transfected lysate ( 39.49 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-QTRT1 full-length
Host
Human
Theoretical MW (kDa)
39.49
Interspecies Antigen Sequence
Rat (88)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-QTRT1 antibody (H00081890-B01) by Western Blots.
SDS-PAGE Gel
QTRT1 transfected lysate.
Western Blot
Lane 1: QTRT1 transfected lysate ( 39.49 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — QTRT1
Entrez GeneID
81890GeneBank Accession#
BC015350.1Protein Accession#
AAH15350.1Gene Name
QTRT1
Gene Alias
FP3235, TGT
Gene Description
queuine tRNA-ribosyltransferase 1
Omim ID
609615Gene Ontology
HyperlinkGene Summary
tRNA-guanine transglycosylase (TGT; EC 2.4.2.29) synthesizes queuosine (Q), which is found in tRNAs that recognize NAU and NAC codons, encoding tyr, asn, asp, and his. Prokaryotic TGT is a single protein of 43 kD. In contrast, mammalian TGT appears to be a heterodimer consisting of a 60-kD subunit (USP14; MIM 607274) and a 43-kD catalytic subunit (QTRT1) (Deshpande and Katze, 2001 [PubMed 11255023]).[supplied by OMIM
Other Designations
tRNA-guanine transglycosylase
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