SHARPIN 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SHARPIN full-length
Host
Human
Theoretical MW (kDa)
35.97
Interspecies Antigen Sequence
Mouse (73); Rat (73)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SHARPIN antibody (H00081858-B01) by Western Blots.
SDS-PAGE Gel
SHARPIN transfected lysate.
Western Blot
Lane 1: SHARPIN transfected lysate ( 35.97 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SHARPIN
Entrez GeneID
81858GeneBank Accession#
BC025244.1Protein Accession#
AAH25244.1Gene Name
SHARPIN
Gene Alias
DKFZp434N1923, SIPL1
Gene Description
SHANK-associated RH domain interactor
Gene Ontology
HyperlinkOther Designations
shank-interacting protein-like 1
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Interactome
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Disease
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