NUAK2 monoclonal antibody (M04), clone 2F9
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant NUAK2.
Immunogen
NUAK2 (NP_112214.1, 521 a.a. ~ 628 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
FGSLDELAPPRPLARASRPSGAVSEDSILSSESFDQLDLPERLPEPPLRGCVSVDNLTGLEEPPSEGPGSCLRRWRQDPLGDSCFSLTDCQEVTATYRQALRVCSKLT
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (84); Rat (84)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.62 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of NUAK2 expression in transfected 293T cell line by NUAK2 monoclonal antibody (M04), clone 2F9.
Lane 1: NUAK2 transfected lysate (Predicted MW: 69.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NUAK2 is 1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to NUAK2 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — NUAK2
Entrez GeneID
81788GeneBank Accession#
NM_030952Protein Accession#
NP_112214.1Gene Name
NUAK2
Gene Alias
DKFZp434J037, DKFZp686F01113, FLJ90349, SNARK
Gene Description
NUAK family, SNF1-like kinase, 2
Omim ID
608131Gene Ontology
HyperlinkGene Summary
SNF1-like kinase
Other Designations
OTTHUMP00000035319|SNF1/AMP activated protein kinase
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