DIAPH3 monoclonal antibody (M01), clone 4D5
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant DIAPH3.
Immunogen
DIAPH3 (NP_112194, 632 a.a. ~ 729 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
PDILNFVDDLEPLDKASKVSVETLEKNLRQMGRQLQQLEKELETFPPPEDLHDKFVTKMSRFVISAKEQYETLSKLHENMEKLYQSIIGYYAIDVKKV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (85)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.52 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of DIAPH3 expression in transfected 293T cell line by DIAPH3 monoclonal antibody (M01), clone 4D5.
Lane 1: DIAPH3 transfected lysate (Predicted MW: 98.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DIAPH3 is 1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to DIAPH3 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — DIAPH3
Entrez GeneID
81624GeneBank Accession#
NM_030932Protein Accession#
NP_112194Gene Name
DIAPH3
Gene Alias
DKFZp434C0931, DKFZp686A13178, DRF3, Dia2, FLJ34705, diap3
Gene Description
diaphanous homolog 3 (Drosophila)
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000018480|diaphanous homolog 3|diaphanous-related formin 3
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Interactome
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Pathway
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