NDEL1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NDEL1 transfected lysate.
Western Blot
Lane 1: NDEL1 transfected lysate ( 38.40 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NDEL1 full-length
Host
Human
Theoretical MW (kDa)
38.4
Interspecies Antigen Sequence
Mouse (95); Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NDEL1 antibody (H00081565-D01P) by Western Blots.
SDS-PAGE Gel
NDEL1 transfected lysate.
Western Blot
Lane 1: NDEL1 transfected lysate ( 38.40 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NDEL1
Entrez GeneID
81565GeneBank Accession#
NM_030808Protein Accession#
NP_110435.1Gene Name
NDEL1
Gene Alias
DKFZp451M0318, EOPA, MITAP1, NUDEL
Gene Description
nudE nuclear distribution gene E homolog (A. nidulans)-like 1
Omim ID
607538Gene Ontology
HyperlinkGene Summary
This gene encodes a thiol-activated peptidase that is phosphorylated in M phase of the cell cycle. Phosphorylation regulates the cell cycle-dependent distribution of this protein, with a fraction of the protein bound strongly to centrosomes in interphase and localized to mitotic spindles in early M phase. Overall, this protein plays a role in nervous system development. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
NUDE-like protein|endooligopeptidase A|mitosin-associated protein MITAP1|nudE nuclear distribution gene E homolog like 1
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Interactome
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Disease
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