TUBB1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
TUBB1 transfected lysate.
Western Blot
Lane 1: TUBB1 transfected lysate ( 50.30 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TUBB1 full-length
Host
Human
Theoretical MW (kDa)
50.3
Interspecies Antigen Sequence
Mouse (91); Rat (91)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TUBB1 antibody (H00081027-D01P) by Western Blots.
SDS-PAGE Gel
TUBB1 transfected lysate.
Western Blot
Lane 1: TUBB1 transfected lysate ( 50.30 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TUBB1
Entrez GeneID
81027GeneBank Accession#
NM_030773Protein Accession#
NP_110400.1Gene Name
TUBB1
Gene Alias
dJ543J19.4
Gene Description
tubulin, beta 1
Gene Ontology
HyperlinkGene Summary
Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, platelet formation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that is wound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in platelets and megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 [PubMed 3782288]; Schulze et al., 2004 [PubMed 15315966]).[supplied by OMIM
Other Designations
OTTHUMP00000031411|beta tubulin 1, class VI
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Interactome
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Pathway
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