CD276 (Human) Recombinant Protein
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Specification
Product Description
Purified CD276 (AAH62581.1 237 a.a. - 461 a.a.) human recombinant protein with His-Flag-StrepII tag at N-terminus expressed in human cells.
Transfected Cell Line
Human HEK293H cells
Sequence
ITPQRSPTGAVEVQVPEDPVVALVGTDATLRCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFTEGRDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYRGYPEAEVFWQDGQGVPLTGNVTTSQMANEQGLFDVHSVLRVVLGANGTYSCLVRNPVLQQDAHGSVTITGQPMT
Host
Human
Theoretical MW (kDa)
30.03
Interspecies Antigen Sequence
Mouse (90); Rat (84)
Form
Liquid
Preparation Method
Transfection of pSuper-CD276 plasmid into HEK293H cell, and the expressed protein was purified by Strep-Tactin affinity column.
Purification
Strep-Tactin affinity columns
Concentration
≥ 10 ug/ml
Storage Buffer
100 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM EDTA, and 5 mM desthiobiotin.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
SDS-PAGE
Protein Interaction
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Gene Info — CD276
Entrez GeneID
80381GeneBank Accession#
BC062581.1Protein Accession#
AAH62581.1Gene Name
CD276
Gene Alias
B7-H3, B7H3
Gene Description
CD276 molecule
Omim ID
605715Gene Ontology
HyperlinkGene Summary
Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558).[supplied by OMIM
Other Designations
B7 homolog 3|CD276 antigen
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Interactome
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Pathway
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