COASY 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-COASY full-length
Host
Human
Theoretical MW (kDa)
29.7
Interspecies Antigen Sequence
Rat (85)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-COASY antibody (H00080347-B01) by Western Blots.
SDS-PAGE Gel
COASY transfected lysate.
Western Blot
Lane 1: COASY transfected lysate ( 29.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — COASY
Entrez GeneID
80347GeneBank Accession#
NM_001042531.1Protein Accession#
-Gene Name
COASY
Gene Alias
DPCK, FLJ35179, NBP, PPAT, UKR1, pOV-2
Gene Description
Coenzyme A synthase
Omim ID
609855Gene Ontology
HyperlinkGene Summary
Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM
Other Designations
bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase|coenzyme A synthase|nucleotide binding protein|phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase
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Interactome
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Pathway
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Disease
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