Western blot analysis of COASY over-expressed 293 cell line, cotransfected with COASY Validated Chimera RNAi ( Cat # H00080347-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with COASY monoclonal antibody (M01), clone 1H6 (Cat # H00080347-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 188.8.131.52) and dephospho-CoA kinase (DPCK; EC 184.108.40.206), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM
bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase,coenzyme A synthase,nucleotide binding protein,phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase