COASY monoclonal antibody (M01), clone 1H6
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More Files
- More Functions
Western Blot (Cell lysate)
COASY monoclonal antibody (M01), clone 1H6 Western Blot analysis of COASY expression in A-431 ( Cat # L015V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of COASY expression in transfected 293T cell line by COASY monoclonal antibody (M01), clone 1H6.
Lane 1: COASY transfected lysate(30.2 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged COASY is approximately 0.1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of COASY over-expressed 293 cell line, cotransfected with COASY Validated Chimera RNAi ( Cat # H00080347-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with COASY monoclonal antibody (M01), clone 1H6 (Cat # H00080347-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (50.49 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant COASY.
Immunogen
COASY (AAH06354, 1 a.a. ~ 225 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MLGNLLRPPYERPELPTCLYVIGLTGISGSGKSSIAQRLKGLGAFVIDSDHLGHRAYAPGGPAYQPVVEAFGTDILHKDGIINRKVLGSRVFGNKKQLKILTDIMWPIIAKLAREEMDRAVAEGKRVCVIDAAVLLEAGWQNLVHEVWTAVIPETEAVRRIVERDGLSEAAAQSRLQSQMSGQQLVEQSHVVLSTLWEPHITQRQVEKAWALLQKRIPKTHQALD
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Rat (85)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (50.49 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
COASY monoclonal antibody (M01), clone 1H6 Western Blot analysis of COASY expression in A-431 ( Cat # L015V1 ).Western Blot (Transfected lysate)
Western Blot analysis of COASY expression in transfected 293T cell line by COASY monoclonal antibody (M01), clone 1H6.
Lane 1: COASY transfected lysate(30.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged COASY is approximately 0.1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of COASY over-expressed 293 cell line, cotransfected with COASY Validated Chimera RNAi ( Cat # H00080347-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with COASY monoclonal antibody (M01), clone 1H6 (Cat # H00080347-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — COASY
Entrez GeneID
80347GeneBank Accession#
BC006354Protein Accession#
AAH06354Gene Name
COASY
Gene Alias
DPCK, FLJ35179, NBP, PPAT, UKR1, pOV-2
Gene Description
Coenzyme A synthase
Omim ID
609855Gene Ontology
HyperlinkGene Summary
Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM
Other Designations
bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase|coenzyme A synthase|nucleotide binding protein|phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase
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Interactome
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Pathway
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Disease
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