This antibody pair set comes with matched antibody pair to detect and quantify protein level of human COASY.
Quality Control Testing:
Standard curve using recombinant protein ( H00080347-P01 ) as an analyte.
Sandwich ELISA detection sensitivity ranging from 3 ng/ml to 100 ng/ml.
Antibody pair set content: 1. Capture antibody: rabbit MaxPab® affinity purified polyclonal anti-COASY (100 ug) 2. Detection antibody: mouse monoclonal anti-COASY, IgG1 Kappa (20 ug) *Reagents are sufficient for at least 1-2 x 96 well plates using recommended protocols.
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 184.108.40.206) and dephospho-CoA kinase (DPCK; EC 220.127.116.11), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM
bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase,coenzyme A synthase,nucleotide binding protein,phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase