PUS1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PUS1 transfected lysate
Western Blot
Lane 1: PUS1 transfected lysate ( 44 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PUS1 full-length
Host
Human
Theoretical MW (kDa)
44
Interspecies Antigen Sequence
Mouse (82); Rat (86)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PUS1 antibody (H00080324-B01) by Western Blots.
SDS-PAGE Gel
PUS1 transfected lysate
Western Blot
Lane 1: PUS1 transfected lysate ( 44 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PUS1
Entrez GeneID
80324GeneBank Accession#
BC002901Protein Accession#
AAH02901Gene Name
PUS1
Gene Alias
MGC11268, MLASA
Gene Description
pseudouridylate synthase 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia. Alternate splicing results in multiple transcript variants
Other Designations
pseudouridine synthase 1
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