RNF34 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RNF34 transfected lysate.
Western Blot
Lane 1: RNF34 transfected lysate ( 41.14 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RNF34 full-length
Host
Human
Theoretical MW (kDa)
41.14
Interspecies Antigen Sequence
Mouse (88); Rat (85)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RNF34 antibody (H00080196-B01) by Western Blots.
SDS-PAGE Gel
RNF34 transfected lysate.
Western Blot
Lane 1: RNF34 transfected lysate ( 41.14 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RNF34
Entrez GeneID
80196GeneBank Accession#
NM_194271.1Protein Accession#
NP_919247.1Gene Name
RNF34
Gene Alias
FLJ21786, RFI, RIF, RIFF
Gene Description
ring finger protein 34
Omim ID
608299Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
FYVE-RING finger protein MOMO|RING finger protein RIFF
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