CENPT 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CENPT transfected lysate.
Western Blot
Lane 1: CENPT transfected lysate ( 32.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CENPT full-length
Host
Human
Theoretical MW (kDa)
32.2
Interspecies Antigen Sequence
Mouse (58); Rat (56)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CENPT antibody (H00080152-B01) by Western Blots.
SDS-PAGE Gel
CENPT transfected lysate.
Western Blot
Lane 1: CENPT transfected lysate ( 32.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CENPT
Entrez GeneID
80152GeneBank Accession#
NM_025082Protein Accession#
NP_079358Gene Name
CENPT
Gene Alias
C16orf56, CENP-T, FLJ13111, FLJ43376
Gene Description
centromere protein T
Omim ID
611510Gene Ontology
HyperlinkGene Summary
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPT is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM
Other Designations
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