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SEMA6D 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00080031-T02
  • Visit Frequency :
  • Countries :
  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-SEMA6D full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 54.2
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-SEMA6Dantibody (H00080031-D01P)byWesternBlots.
    SDS-PAGE Gel
    QC Testing of H00080031-T02
    SEMA6D transfected lysate.
    Western Blot
    QC Testing of H00080031-T02
    Lane 1: SEMA6D transfected lysate ( 54.2 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • SEMA6D
  • Gene Alias:
  • FLJ11598,KIAA1479
  • Gene Description:
  • sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
  • Gene Summary:
  • Semaphorins are a large family, including both secreted and membrane associated proteins, many of which have been implicated as inhibitors or chemorepellents in axon pathfinding, fasciculation and branching, and target selection. All semaphorins possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Additional sequence motifs C-terminal to the semaphorin domain allow classification into distinct subfamilies. Results demonstrate that transmembrane semaphorins, like the secreted ones, can act as repulsive axon guidance cues. This gene encodes a class 6 vertebrate transmembrane semaphorin that demonstrates alternative splicing. Six transcript variants have been identified and expression of the distinct encoded isoforms is thought to be regulated in a tissue- and development-dependent manner. [provided by RefSeq
  • Other Designations:
  • semaphorin 6D
  • Gene Pathway
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