SEMA6D 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
SEMA6D transfected lysate.
Western Blot
Lane 1: SEMA6D transfected lysate ( 54.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SEMA6D full-length
Host
Human
Theoretical MW (kDa)
54.2
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SEMA6Dantibody (H00080031-D01P)byWesternBlots.
SDS-PAGE Gel
SEMA6D transfected lysate.
Western Blot
Lane 1: SEMA6D transfected lysate ( 54.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SEMA6D
Entrez GeneID
80031GeneBank Accession#
NM_024966.2Protein Accession#
NP_079242.2Gene Name
SEMA6D
Gene Alias
FLJ11598, KIAA1479
Gene Description
sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
Omim ID
609295Gene Ontology
HyperlinkGene Summary
Semaphorins are a large family, including both secreted and membrane associated proteins, many of which have been implicated as inhibitors or chemorepellents in axon pathfinding, fasciculation and branching, and target selection. All semaphorins possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Additional sequence motifs C-terminal to the semaphorin domain allow classification into distinct subfamilies. Results demonstrate that transmembrane semaphorins, like the secreted ones, can act as repulsive axon guidance cues. This gene encodes a class 6 vertebrate transmembrane semaphorin that demonstrates alternative splicing. Six transcript variants have been identified and expression of the distinct encoded isoforms is thought to be regulated in a tissue- and development-dependent manner. [provided by RefSeq
Other Designations
semaphorin 6D
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Pathway
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Disease
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