FBXL18 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human FBXL18 full-length ORF ( AAH13435.1, 1 a.a. - 259 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MHAVPRGFGKKVRVGVQSCPPLLGPGVPPALLRVLVSAEEPALPGTPRADWVQLLLRHAPQRARHPQLAPTLQPRTECRGLGGGRHRPAGLPAAPDARTAAQRPYGLRAGQYRPAVPAVAVPVAGQPGHDGEGGVHARALRHVEALQAAEGPQAGAALLQRQRPVLPGAEPVPLAAAPVPGLSQRHPPARCRAGLHGSLPAGCHVPPVHRGVPRHLQEPAAVASPQLPGRAARVKRRHLPSAPRGPDRRHPGRPPGAPG
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
53.3
Interspecies Antigen Sequence
Mouse (72); Rat (72)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — FBXL18
Entrez GeneID
80028GeneBank Accession#
BC013435.1Protein Accession#
AAH13435.1Gene Name
FBXL18
Gene Alias
FLJ10776, FLJ11467, FLJ26934, FLJ38075, FLJ41541, Fbl18
Gene Description
F-box and leucine-rich repeat protein 18
Omim ID
609084Gene Ontology
HyperlinkGene Summary
Members of the F-box protein family, such as FBXL18, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM
Other Designations
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Interactome
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Publication Reference
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An engineered platform for reconstituting functional multisubunit SCF E3 ligase in vitro.
Huihui Liu, Simiao Liu, Hong Yu, Xiahe Huang, Yingchun Wang, Liang Jiang, Xiangbing Meng, Guifu Liu, Mingjiang Chen, Yanhui Jing, Feifei Yu, Bing Wang, Jiayang Li.
Molecular Plant 2022 Aug; 15(8):1285.
Application:enzyme reconstitution, Human, E3 ligase.
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An engineered platform for reconstituting functional multisubunit SCF E3 ligase in vitro.
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