NEK11 monoclonal antibody (M01), clone 4E1-1F4
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant NEK11.
Immunogen
NEK11 (AAH28587, 1 a.a. ~ 482 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MLKFQEAAKCVSGSTAISTYPKTLIARRYVLQQKLGSGSFGTVYLVSDKKAKRGEELKVLKEISVGELNPNETVQANLEAQLLSKLDHPAIVKFHASFVEQDNFCIITEYCEGRDLDDKIQEYKQAGKIFPENQIIEWFIQLLLGVDYMHERRILHRDLKSKNVFLKNNLLKIGDFGVSRLLMGSCDLATTLTGTPHYMSPEALKHQGYDTKSDIWSLACILYEMCCMNHAFAGSNFLSIVLKIVEGDTPSLPERYPKELNAIMESMLNKNPSLRPSAIEILKIPYLDEQLQNLMCRYSEMTLEDKNLDCQKEAAHIINAMQKRIHLQTLRALSEVQKMTPRERMRLRKLQAADEKARKLKKIVEEKYEENSKRMQELRSRNFQQLSVDVLHEKTHLKGMEEKEEQPEGRLSCSPQDEDEERWQGREEESDEPTLENLPESQPIPSMDLHELESIVEDATSDLGYHGDCNLISLDEYWKNEK
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (73); Rat (70)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (78.54 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
NEK11 monoclonal antibody (M01), clone 4E1-1F4. Western Blot analysis of NEK11 expression in A-431 ( Cat # L015V1 ).Western Blot (Cell lysate)
NEK11 monoclonal antibody (M01), clone 4E1-1F4 Western Blot analysis of NEK11 expression in HL-60 ( Cat # L014V1 ).Western Blot (Transfected lysate)
Western Blot analysis of NEK11 expression in transfected 293T cell line by NEK11 monoclonal antibody (M01), clone 4E1-1F4.
Lane 1: NEK11 transfected lysate(55.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to NEK11 on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NEK11 is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to NEK11 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — NEK11
Entrez GeneID
79858GeneBank Accession#
BC028587Protein Accession#
AAH28587Gene Name
NEK11
Gene Alias
FLJ23495
Gene Description
NIMA (never in mitosis gene a)- related kinase 11
Omim ID
609779Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described
Other Designations
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Interactome
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Disease
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