PDZD3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PDZD3 transfected lysate.
Western Blot
Lane 1: PDZD3 transfected lysate ( 52.50 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PDZD3 full-length
Host
Human
Theoretical MW (kDa)
52.5
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PDZD3 antibody (H00079849-D01P) by Western Blots.
SDS-PAGE Gel
PDZD3 transfected lysate.
Western Blot
Lane 1: PDZD3 transfected lysate ( 52.50 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PDZD3
Entrez GeneID
79849GeneBank Accession#
NM_024791Protein Accession#
NP_079067.2Gene Name
PDZD3
Gene Alias
FLJ22756, IKEPP, PDZK2
Gene Description
PDZ domain containing 3
Omim ID
607146Gene Ontology
HyperlinkGene Summary
Guanylyl cyclase C (GCC, or GUCY2C; MIM 601330) produces cGMP following the binding of either endogenous ligands or heat-stable enterotoxins secreted by E. coli and other enteric bacteria. Activation of GCC initiates a signaling cascade that leads to phosphorylation of the cystic fibrosis transmembrane conductance regulator (CFTR; MIM 602421), followed by a net efflux of ions and water into the intestinal lumen. IKEPP is a regulatory protein that associates with GCC and regulates the amount of cGMP produced following receptor stimulation (Scott et al., 2002 [PubMed 11950846]).[supplied by OMIM
Other Designations
PDZ domain containing 2|intestinal and kidney enriched PDZ protein|natrium-phosphate cotransporter IIa C-terminal-associated protein 2
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