NHEJ1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NHEJ1 transfected lysate.
Western Blot
Lane 1: NHEJ1 transfected lysate ( 33 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NHEJ1 full-length
Host
Human
Theoretical MW (kDa)
33
Interspecies Antigen Sequence
Mouse (73); Rat (75)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NHEJ1 antibody (H00079840-B01) by Western Blots.
SDS-PAGE Gel
NHEJ1 transfected lysate.
Western Blot
Lane 1: NHEJ1 transfected lysate ( 33 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NHEJ1
Entrez GeneID
79840GeneBank Accession#
NM_024782.1Protein Accession#
NP_079058.1Gene Name
NHEJ1
Gene Alias
FLJ12610, XLF
Gene Description
nonhomologous end-joining factor 1
Omim ID
611290Gene Ontology
HyperlinkGene Summary
Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq
Other Designations
Cernunnos|XRCC4-like factor
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Interactome
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Pathway
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Disease
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