FBXO31 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
FBXO31 transfected lysate.
Western Blot
Lane 1: FBXO31 transfected lysate ( 40.48 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-FBXO31 full-length
Host
Human
Theoretical MW (kDa)
40.48
Interspecies Antigen Sequence
Mouse (85); Rat (84)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FBXO31 antibody (H00079791-B01) by Western Blots.
SDS-PAGE Gel
FBXO31 transfected lysate.
Western Blot
Lane 1: FBXO31 transfected lysate ( 40.48 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — FBXO31
Entrez GeneID
79791GeneBank Accession#
BC012748.1Protein Accession#
AAH12748.1Gene Name
FBXO31
Gene Alias
DKFZp434B027, DKFZp434J1815, FBX14, FBXO14, FLJ22477, Fbx31, MGC15419, MGC9527, pp2386
Gene Description
F-box protein 31
Omim ID
609102Gene Ontology
HyperlinkGene Summary
Members of the F-box protein family, such as FBXO31, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM
Other Designations
F-box only protein 31|SCF ubiquitin ligase specificity factor|putative breast cancer tumor-suppressor
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