GALNT12 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GALNT12 full-length
Host
Human
Theoretical MW (kDa)
30.03
Interspecies Antigen Sequence
Mouse (83); Rat (81)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GALNT12 antibody (H00079695-B02) by Western Blots.
SDS-PAGE Gel
GALNT12 transfected lysate.
Western Blot
Lane 1: GALNT12 transfected lysate ( 30.03 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GALNT12
Entrez GeneID
79695GeneBank Accession#
BC013945Protein Accession#
AAH13945Gene Name
GALNT12
Gene Alias
FLJ21212, GalNAc-T12
Gene Description
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 12 (GalNAc-T12)
Omim ID
610290Gene Ontology
HyperlinkGene Summary
GALNT12 is a member of a family of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (EC 2.4.1.41), which catalyze the transfer of N-acetylgalactosamine (GalNAc) from UDP-GalNAc to a hydroxyl amino acid on a polypeptide acceptor in the initial step of mucin-type O-linked protein glycosylation (Guo et al., 2002 [PubMed 12135769]).[supplied by OMIM
Other Designations
OTTHUMP00000021781|UDP-GalNAc: polypeptide N-acetylgalactosaminyltransferase|polypeptide N-acetylgalactosaminyltransferase 12
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