WDR58 monoclonal antibody (M01), clone 1F6
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More Files
- More Functions
Western Blot (Cell lysate)
WDR58 monoclonal antibody (M01), clone 1F6. Western Blot analysis of WDR58 expression in PC-12 ( Cat # L012V1 ).
Western Blot (Cell lysate)
WDR58 monoclonal antibody (M01), clone 1F6 Western Blot analysis of WDR58 expression in A-431 ( Cat # L015V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of THOC6 expression in transfected 293T cell line by THOC6 monoclonal antibody (M01), clone 1F6.
Lane 1: THOC6 transfected lysate (Predicted MW: 37.5 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to WDR58 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged WDR58 is approximately 0.3ng/ml as a capture antibody.
Western Blot detection against Immunogen (37.84 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant WDR58.
Immunogen
WDR58 (NP_077315, 71 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
TFQAHDGPVYSMVSTDRHLLSAGDGEVKAWLWAEMLKKGCKELWRRQPPYRTSLEVPEINALLLVPKENSLILAGGDCQLHTMDLETGTFTRVLRGHTDYIHCLALRERS
Host
Mouse
Reactivity
Human, Rat
Interspecies Antigen Sequence
Mouse (95); Rat (95)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
WDR58 monoclonal antibody (M01), clone 1F6. Western Blot analysis of WDR58 expression in PC-12 ( Cat # L012V1 ).Western Blot (Cell lysate)
WDR58 monoclonal antibody (M01), clone 1F6 Western Blot analysis of WDR58 expression in A-431 ( Cat # L015V1 ).Western Blot (Transfected lysate)
Western Blot analysis of THOC6 expression in transfected 293T cell line by THOC6 monoclonal antibody (M01), clone 1F6.
Lane 1: THOC6 transfected lysate (Predicted MW: 37.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to WDR58 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged WDR58 is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — THOC6
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Interactome
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Disease
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Publication Reference
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Human TREX component Thoc5 affects alternative polyadenylation site choice by recruiting mammalian cleavage factor I.
Katahira J, Okuzaki D, Inoue H, Yoneda Y, Maehara K, Ohkawa Y.
Nucleic Acids Research 2013 Aug; 41(14):7060.
Application：WB-Ce, Human, HeLa cells.
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Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA.
Katahira J, Inoue H, Hurt E, Yoneda Y.
The EMBO Journal 2009 Mar; 28(5):556.
Application：WB, Human, HeLa cells.
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Human TREX component Thoc5 affects alternative polyadenylation site choice by recruiting mammalian cleavage factor I.
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