IRX6 monoclonal antibody (M01), clone 1A5
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant IRX6.
Immunogen
IRX6 (NP_077311, 337 a.a. ~ 446 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
PRLTMHYPCLEKPRIWSLAHTATASAVEGAPPARPRPRSPECRMIPGQPPASARRLSVPRDSACDESSCIPKAFGNPKFALQGLPLNCAPCPRRSEPVVQCQYPSGAEAG
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (75); Rat (76)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of IRX6 expression in transfected 293T cell line by IRX6 monoclonal antibody (M01), clone 1A5.
Lane 1: IRX6 transfected lysate(48.169 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged IRX6 is 0.1 ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of IRX6 over-expressed 293 cell line, cotransfected with IRX6 Validated Chimera RNAi ( Cat # H00079190-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IRX6 monoclonal antibody (M01), clone 1A5 (Cat # H00079190-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — IRX6
Entrez GeneID
79190GeneBank Accession#
NM_024335Protein Accession#
NP_077311Gene Name
IRX6
Gene Alias
IRX-3, IRX7, IRXB3
Gene Description
iroquois homeobox 6
Omim ID
606196Gene Ontology
HyperlinkOther Designations
iroquois homeobox protein 1-like|iroquois homeobox protein 6|iroquois homeobox protein 7
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Interactome
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