FA2H 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-FA2H full-length
Host
Human
Theoretical MW (kDa)
41.03
Interspecies Antigen Sequence
Mouse (82); Rat (81)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FA2H antibody (H00079152-B01) by Western Blots.
SDS-PAGE Gel
FA2H transfected lysate.
Western Blot
Lane 1: FA2H transfected lysate ( 41.03 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — FA2H
Entrez GeneID
79152GeneBank Accession#
BC017049.1Protein Accession#
AAH17049.2Gene Name
FA2H
Gene Alias
FAAH, FAH1, FAXDC1, FLJ25287, SCS7
Gene Description
fatty acid 2-hydroxylase
Omim ID
611026Gene Ontology
HyperlinkGene Summary
Sphingolipids are a large class of lipids found in all eukaryotic cells and are involved in numerous cellular processes. The structural diversity of sphingolipids stems from more than 300 distinct head groups, as well as from modifications of the hydrophobic ceramide moiety. FA2H catalyzes a common modification of the ceramide moiety: hydroxylation at the 2 position of the N-acyl chain. Sphingolipids containing 2-hydroxy fatty acid are common in nervous and epidermal tissue. Glycosphingolipids containing a high proportion of 2-hydroxy fatty acid are critical components of myelin, and several very long chain ceramides with 2-hydroxy fatty acids are important for the permeability barrier function of epidermis (Alderson et al., 2004 [PubMed 15337768]).[supplied by OMIM
Other Designations
fatty acid hydroxylase domain containing 1
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