WDR77 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
WDR77 transfected lysate.
Western Blot
Lane 1: WDR77 transfected lysate ( 37.73 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-WDR77 full-length
Host
Human
Theoretical MW (kDa)
37.73
Interspecies Antigen Sequence
Mouse (89)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-WDR77 antibody (H00079084-B01) by Western Blots.
SDS-PAGE Gel
WDR77 transfected lysate.
Western Blot
Lane 1: WDR77 transfected lysate ( 37.73 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — WDR77
Entrez GeneID
79084GeneBank Accession#
NM_024102.2Protein Accession#
NP_077007.1Gene Name
WDR77
Gene Alias
HKMT1069, MEP50, MGC2722, Nbla10071, RP11-552M11.3, p44
Gene Description
WD repeat domain 77
Gene Ontology
HyperlinkGene Summary
WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).[supplied by OMIM
Other Designations
OTTHUMP00000013472|androgen receptor cofactor p44|methylosome protein 50
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