WDR77 monoclonal antibody (M01), clone 3F10

Catalog # H00079084-M01

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Size:100 ug
Price: USD $ 335.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

WDR77 monoclonal antibody (M01), clone 3F10 Western Blot analysis of WDR77 expression in HeLa ( Cat # L013V1 ).

Western Blot (Transfected lysate)
Application

Western Blot (Transfected lysate)

Western Blot analysis of WDR77 expression in transfected 293T cell line by WDR77 monoclonal antibody (M01), clone 3F10.

Lane 1: WDR77 transfected lysate(36.7 KDa).
Lane 2: Non-transfected lysate.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunoperoxidase of monoclonal antibody to WDR77 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

Sandwich ELISA (Recombinant protein)
Application

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged WDR77 is approximately 3ng/ml as a capture antibody.

QC Test

Western Blot detection against Immunogen (63.36 KDa) .

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against a full length recombinant WDR77.

    Immunogen

    WDR77 (AAH01679, 1 a.a. ~ 342 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

    Sequence

    MRKETPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGALLLGASSLSGRCWAGSLWLFKDPCAAPNEGFCSAGVQTEAGVADLTWVGERGILVASDSGAVELWELDENETLIVSKFCKYEHDDIVSTVSVLSSGTQAVSGSKDICIKVWDLAQQVVLSSYRAHAAQVTCVAASPHKDSVFLSCSEDNRILLWDTRCPKPASQIGCSAPGYLPTSLAWHPQQSEVFVFGDENGTVSLVDTKSTSCVLSSAVHSQCVTGLVFSPHSVPFLASLSEDCSLAVLDSSLSELFRSQAHRDFVRDATWSPLNHSLLTTVGWDHQVVHHVVPTEPLPAPGPASVTE

    Host

    Mouse

    Reactivity

    Human

    Interspecies Antigen Sequence

    Mouse (89)

    Isotype

    IgG1 Kappa

    Quality Control Testing

    Antibody Reactive Against Recombinant Protein.

    Western Blot detection against Immunogen (63.36 KDa) .

    Storage Buffer

    In 1x PBS, pH 7.4

    Storage Instruction

    Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot (Cell lysate)

    WDR77 monoclonal antibody (M01), clone 3F10 Western Blot analysis of WDR77 expression in HeLa ( Cat # L013V1 ).

    Western Blot (Transfected lysate)

    Western Blot analysis of WDR77 expression in transfected 293T cell line by WDR77 monoclonal antibody (M01), clone 3F10.

    Lane 1: WDR77 transfected lysate(36.7 KDa).
    Lane 2: Non-transfected lysate.

    Western Blot (Recombinant protein)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunoperoxidase of monoclonal antibody to WDR77 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

    Sandwich ELISA (Recombinant protein)

    Detection limit for recombinant GST tagged WDR77 is approximately 3ng/ml as a capture antibody.

    ELISA

  • Gene Info — WDR77

    Entrez GeneID

    79084

    GeneBank Accession#

    BC001679

    Protein Accession#

    AAH01679

    Gene Name

    WDR77

    Gene Alias

    HKMT1069, MEP50, MGC2722, Nbla10071, RP11-552M11.3, p44

    Gene Description

    WD repeat domain 77

    Gene Ontology

    Hyperlink

    Gene Summary

    WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).[supplied by OMIM

    Other Designations

    OTTHUMP00000013472|androgen receptor cofactor p44|methylosome protein 50

  • Interactomes
  • Publication Reference
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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