WDR77 monoclonal antibody (M01), clone 3F10
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More Files
- More Functions
Western Blot (Cell lysate)
WDR77 monoclonal antibody (M01), clone 3F10 Western Blot analysis of WDR77 expression in HeLa ( Cat # L013V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of WDR77 expression in transfected 293T cell line by WDR77 monoclonal antibody (M01), clone 3F10.
Lane 1: WDR77 transfected lysate(36.7 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to WDR77 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged WDR77 is approximately 3ng/ml as a capture antibody.
Western Blot detection against Immunogen (63.36 KDa) .
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Specifications
Product Description
Mouse monoclonal antibody raised against a full length recombinant WDR77.
Immunogen
WDR77 (AAH01679, 1 a.a. ~ 342 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MRKETPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGALLLGASSLSGRCWAGSLWLFKDPCAAPNEGFCSAGVQTEAGVADLTWVGERGILVASDSGAVELWELDENETLIVSKFCKYEHDDIVSTVSVLSSGTQAVSGSKDICIKVWDLAQQVVLSSYRAHAAQVTCVAASPHKDSVFLSCSEDNRILLWDTRCPKPASQIGCSAPGYLPTSLAWHPQQSEVFVFGDENGTVSLVDTKSTSCVLSSAVHSQCVTGLVFSPHSVPFLASLSEDCSLAVLDSSLSELFRSQAHRDFVRDATWSPLNHSLLTTVGWDHQVVHHVVPTEPLPAPGPASVTE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (89)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (63.36 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
WDR77 monoclonal antibody (M01), clone 3F10 Western Blot analysis of WDR77 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of WDR77 expression in transfected 293T cell line by WDR77 monoclonal antibody (M01), clone 3F10.
Lane 1: WDR77 transfected lysate(36.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to WDR77 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged WDR77 is approximately 3ng/ml as a capture antibody.ELISA
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Gene Info — WDR77
Entrez GeneID
79084GeneBank Accession#
BC001679Protein Accession#
AAH01679Gene Name
WDR77
Gene Alias
HKMT1069, MEP50, MGC2722, Nbla10071, RP11-552M11.3, p44
Gene Description
WD repeat domain 77
Gene Ontology
HyperlinkGene Summary
WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).[supplied by OMIM
Other Designations
OTTHUMP00000013472|androgen receptor cofactor p44|methylosome protein 50
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Interactomes
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Publication Reference
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MEP50/PRMT5-mediated methylation activates GLI1 in Hedgehog signalling through inhibition of ubiquitination by the ITCH/NUMB complex.
Abe Y, Suzuki Y, Kawamura K, Tanaka N.
Communications Biology 2019 Jan; 2:23.
Application：IF, WB-Tr, Human, AGS, C3H10T1/2 cells.
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PRMT5 Is Upregulated in Malignant and Metastatic Melanoma and Regulates Expression of MITF and p27(Kip1).
Nicholas C, Yang J, Peters SB, Bill MA, Baiocchi RA, Yan F, Sif S, Tae S, Gaudio E, Wu X, Grever MR, Young GS, Lesinski GB.
PLoS One 2013 Sep; 8(9):e74710.
Application：IP-WB, WB-Ce, Human, HEM, A375, Hs294T, 1106Mel, FO-1, WM1366, MeWo, CHL-1 cells.
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MEP50/PRMT5-mediated methylation activates GLI1 in Hedgehog signalling through inhibition of ubiquitination by the ITCH/NUMB complex.
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