MLPH 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MLPH transfected lysate.
Western Blot
Lane 1: MLPH transfected lysate ( 65.9 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MLPH full-length
Host
Human
Theoretical MW (kDa)
65.9
Interspecies Antigen Sequence
Mouse (63); Rat (64)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MLPH antibody (H00079083-B01) by Western Blots.
SDS-PAGE Gel
MLPH transfected lysate.
Western Blot
Lane 1: MLPH transfected lysate ( 65.9 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MLPH
Entrez GeneID
79083GeneBank Accession#
NM_024101Protein Accession#
NP_077006Gene Name
MLPH
Gene Alias
MGC2771, MGC59733, SLAC2-A
Gene Description
melanophilin
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the exophilin subfamily of Rab effector proteins. The protein forms a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. A similar protein complex in mouse functions to tether pigment-producing organelles called melanosomes to the actin cytoskeleton in melanocytes, and is required for visible pigmentation in the hair and skin. A mutation in this gene results in Griscelli syndrome type 3, which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq
Other Designations
exophilin-3
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Interactome
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Disease
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