DDX54 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
DDX54 transfected lysate.
Western Blot
Lane 1: DDX54 transfected lysate ( 97.02 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DDX54 full-length
Host
Human
Theoretical MW (kDa)
97.02
Interspecies Antigen Sequence
Mouse (84); Rat (84)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DDX54 antibody (H00079039-B01) by Western Blots.
SDS-PAGE Gel
DDX54 transfected lysate.
Western Blot
Lane 1: DDX54 transfected lysate ( 97.02 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DDX54
Entrez GeneID
79039GeneBank Accession#
NM_024072.3Protein Accession#
NP_076977.3Gene Name
DDX54
Gene Alias
DP97, MGC2835
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
Omim ID
611665Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene. [provided by RefSeq
Other Designations
ATP-dependent RNA helicase|DEAD box helicase 97 KDa
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