CENPM 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CENPM transfected lysate.
Western Blot
Lane 1: CENPM transfected lysate ( 19.7 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CENPM full-length
Host
Human
Theoretical MW (kDa)
19.7
Interspecies Antigen Sequence
Mouse (79); Rat (80)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CENPM antibody (H00079019-B01) by Western Blots.
SDS-PAGE Gel
CENPM transfected lysate.
Western Blot
Lane 1: CENPM transfected lysate ( 19.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CENPM
Entrez GeneID
79019GeneBank Accession#
NM_024053Protein Accession#
NP_076958Gene Name
CENPM
Gene Alias
C22orf18, CENP-M, MGC861, PANE1, bK250D10.2
Gene Description
centromere protein M
Omim ID
610152Gene Ontology
HyperlinkGene Summary
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM
Other Designations
OTTHUMP00000028741|proliferation associated nuclear element 1
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Interactome
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