DDX50 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DDX50 full-length
Host
Human
Theoretical MW (kDa)
81.18
Interspecies Antigen Sequence
Mouse (95); Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DDX50 antibody (H00079009-B01) by Western Blots.
SDS-PAGE Gel
DDX50 transfected lysate.
Western Blot
Lane 1: DDX50 transfected lysate ( 81.18 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DDX50
Entrez GeneID
79009GeneBank Accession#
NM_024045.1Protein Accession#
NP_076950.1Gene Name
DDX50
Gene Alias
GU2, GUB, MGC3199, RH-II/GuB
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
Omim ID
610373Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined. [provided by RefSeq
Other Designations
OTTHUMP00000019711|RNA helicase II/Gu beta|nucleolar protein GU2
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