STK33 monoclonal antibody (M09), clone 1F10
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant STK33.
Immunogen
STK33 (AAH31231, 1 a.a. ~ 514 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MADSGLDKKSTKCPDCSSASQKDVLCVCSSKTRVPPVLVVEMSQTSSIGSAESLISLERKKEKNINRDITSRKDLPSRTSNVERKASQQQWGRGNFTEGKVPHIRIENGAAIEEIYTFGRILGKGSFGIVIEATDKETETKWAIKKVNKEKAGSSAVKLLEREVNILKSVKHEHIIHLEQVFETPKKMYLVMELCEDGELKEILDRKGHFSENETRWIIQSLASAIAYLHNNDIVHRDLKLENIMVKSSLIDDNNEINLNIKVTDFGLAVKKQSRSEAMLQATCGTPIYMAPEVISAHDYSQQCDIWSIGVVMYMLLRGEPPFLASSEGKLFELIRKGELHFENAVWNSISDCAKSVLKQLMKVDPAHRITAKELLDNQWLTGNKLSSVRPTNVLEMMKEWKNNPESVEENTTEEKNKPSTEEKLKSYQPWGNVPETNYTSDEEEEKQSTTYEKQFPATSKDNFDMCSSSFTSSKLLPAEIKGEMEKTPVTPSQGTATKYPAKSGALSRTKKKL
Host
Mouse
Reactivity
Human, Mouse, Rat
Interspecies Antigen Sequence
Mouse (68)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (82.28 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
STK33 monoclonal antibody (M09), clone 1F10. Western Blot analysis of STK33 expression in Raw 264.7(Cat # L024V1 ).Western Blot (Cell lysate)
STK33 monoclonal antibody (M09), clone 1F10. Western Blot analysis of STK33 expression in PC-12(Cat # L012V1 ).Western Blot (Cell lysate)
STK33 monoclonal antibody (M09), clone 1F10 Western Blot analysis of STK33 expression in A-431 ( Cat # L015V1 ).Western Blot (Cell lysate)
STK33 monoclonal antibody (M09), clone 1F10. Western Blot analysis of STK33 expression in NIH/3T3(Cat # L018V1 ).Western Blot (Transfected lysate)
Western Blot analysis of STK33 expression in transfected 293T cell line by STK33 monoclonal antibody (M09), clone 1F10.
Lane 1: STK33 transfected lysate(57.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of STK33 transfected lysate using anti-STK33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STK33 MaxPab rabbit polyclonal antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to STK33 on A-431 cell. [antibody concentration 10 ug/ml] -
Gene Info — STK33
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Interactome
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Disease
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Publication Reference
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STK33 Kinase Activity is Non-Essential in KRAS-Dependent Cancer Cells.
Babij C, Zhang Y, Kurzeja RJ, Munzli A, Shehabeldin A, Fernando M, Quon K, Kassner PD, Ruefli-Brasse AA, Watson VJ, Fajardo F, Jackson A, Zondlo J, Sun Y, Ellison AR, Plewa CA, San Miguel T, Robinson J, McCarter J, Schwandner R, Judd T, Carnahan J, Dussault I.
Cancer Res 2011 Jul; 71:5818.
Application:WB-Tr, Human, NOMO-1, SKM-1, DLD-1 cells.
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Synthetic lethal interaction between oncogenic KRAS dependency and STK33 suppression in human cancer cells.
Scholl C, Frohling S, Dunn IF, Schinzel AC, Barbie DA, Kim SY, Silver SJ, Tamayo P, Wadlow RC, Ramaswamy S, Dohner K, Bullinger L, Sandy P, Boehm JS, Root DE, Jacks T, Hahn WC, Gilliland DG.
Cell 2009 May; 137(5):821.
Application:IP, WB-Tr, Human, SKM-1 cells.
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STK33 Kinase Activity is Non-Essential in KRAS-Dependent Cancer Cells.
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