NADK monoclonal antibody (M01), clone 5F4
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
NADK monoclonal antibody (M01), clone 5F4. Western Blot analysis of NADK expression in NIH/3T3 ( Cat # L018V1 ).
Western Blot (Cell lysate)
NADK monoclonal antibody (M01), clone 5F4. Western Blot analysis of NADK expression in HepG2 ( Cat # L019V1 ).
Western Blot (Cell lysate)
NADK monoclonal antibody (M01), clone 5F4 Western Blot analysis of NADK expression in HeLa ( Cat # L013V1 ).
Western Blot (Cell lysate)
NADK monoclonal antibody (M01), clone 5F4. Western Blot analysis of NADK expression in Raw 264.7 ( Cat # L024V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of NADK expression in transfected 293T cell line by NADK monoclonal antibody (M01), clone 5F4.
Lane 1: NADK transfected lysate(49.2 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NADK is approximately 0.3ng/ml as a capture antibody.
Western Blot detection against Immunogen (74.8 KDa) .
-
Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant NADK.
Immunogen
NADK (AAH01709, 1 a.a. ~ 446 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEMEQEKMTMNKELSPDAAAYCCSACHGDETWSYNHPIRGRAKSRSLSASPALGSTKEFRRTRSLHGPCPVTTFGPKACVLQNPQTIMHIQDPASQRLTWNKSPKSVLVIKKMRDASLLQPFKELCTHLMEENMIVYVEKKVLEDPAIASDESFGAVKKKFCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLGFLTPFSFENFQSQVTQVIEGNAAVVLRSRLKVRVVKELRGKKTAVHNGLGEKGSQAAGLDMDVGKQAMQYQVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDGVIVSTPTGSTAYAAAAGASMIHPNVPAIMITPICPHSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGRKRQEIRHGDSISITTSCYPLPSICVRDPVSDWFESLAQCLHWNVRKKQAHFEEEEEEEEEG
Host
Mouse
Reactivity
Human, Mouse
Interspecies Antigen Sequence
Mouse (91); Rat (91)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (74.8 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot (Cell lysate)
NADK monoclonal antibody (M01), clone 5F4. Western Blot analysis of NADK expression in NIH/3T3 ( Cat # L018V1 ).Western Blot (Cell lysate)
NADK monoclonal antibody (M01), clone 5F4. Western Blot analysis of NADK expression in HepG2 ( Cat # L019V1 ).Western Blot (Cell lysate)
NADK monoclonal antibody (M01), clone 5F4 Western Blot analysis of NADK expression in HeLa ( Cat # L013V1 ).Western Blot (Cell lysate)
NADK monoclonal antibody (M01), clone 5F4. Western Blot analysis of NADK expression in Raw 264.7 ( Cat # L024V1 ).Western Blot (Transfected lysate)
Western Blot analysis of NADK expression in transfected 293T cell line by NADK monoclonal antibody (M01), clone 5F4.
Lane 1: NADK transfected lysate(49.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NADK is approximately 0.3ng/ml as a capture antibody.ELISA
-
Gene Info — NADK
Entrez GeneID
65220GeneBank Accession#
BC001709Protein Accession#
AAH01709Gene Name
NADK
Gene Alias
FLJ13052, FLJ37724, dJ283E3.1
Gene Description
NAD kinase
Omim ID
611616Gene Ontology
HyperlinkGene Summary
NADK catalyzes the transfer of a phosphate group from ATP to NAD to generate NADP, which in its reduced form acts as an electron donor for biosynthetic reactions (Lerner et al., 2001 [PubMed 11594753]).[supplied by OMIM
Other Designations
OTTHUMP00000001110|OTTHUMP00000001111
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
NAD kinase levels control the NADPH concentration in human cells.
Pollak N, Niere M, Ziegler M.
The Journal of Biological Chemistry 2007 Sep; 282(46):33562.
Application：IF, WB, Human, HEK 293 cells.
-
NAD kinase levels control the NADPH concentration in human cells.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com