NOL6 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NOL6 full-length
Host
Human
Theoretical MW (kDa)
127.3
Interspecies Antigen Sequence
Mouse (84); Rat (86)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NOL6 antibody (H00065083-B02) by Western Blots.
SDS-PAGE Gel
NOL6 transfected lysate.
Western Blot
Lane 1: NOL6 transfected lysate ( 127.3 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NOL6
Entrez GeneID
65083GeneBank Accession#
BC030139Protein Accession#
AAH30139Gene Name
NOL6
Gene Alias
FLJ21959, MGC14896, MGC14921, MGC20838, NRAP, UTP22, bA311H10.1
Gene Description
nucleolar protein family 6 (RNA-associated)
Omim ID
611532Gene Ontology
HyperlinkGene Summary
The nucleolus is a dense subnuclear membraneless organelle that assembles around clusters of rRNA genes and functions in ribosome biogenesis. This gene encodes a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. Alternative splicing has been observed at this locus and two splice variants encoding distinct isoforms have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000000449|OTTHUMP00000000450|OTTHUMP00000000451|OTTHUMP00000000452|nucleolar protein family 6
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